nf-core/oncoanalyser
A comprehensive cancer DNA/RNA analysis and reporting pipeline
Define where the pipeline should find input data and save output data.
Path to comma-separated file containing information about the samples in the experiment.
string
^\S+\.csv$
You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See usage docs.
The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.
string
Other options specific to this pipeline.
Email address for completion summary.
string
^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$
Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (~/.nextflow/config
) then you don't need to specify this on the command line for every run.
Workflow run mode.
string
^(wgts|targeted)
Name of panel to use.
string
Skip check for restricted genome.
boolean
Run only processes manually provided in processes_include.
boolean
Pipeline processes to exclude.
string
Pipeline processes to include.
string
Prepare and write reference output only.
boolean
Create placeholders for reference data during stub run.
boolean
When positive, will use fastp to split fastq files so that each resultant fastq file has no more than max_fastq_records records. When nonpositive, fastp is not used and the provided fastq files are passed as-is to the aligner.
integer
Path to GRIDSS configuration file.
string
User defined RNA read length used for Isofox.
integer
User defined Isofox expected GC ratios file.
string
User defined Isofox expected counts files (read length dependent).
string
User defined Isofox TPM normalisation file for panel data.
string
User defined Isofox gene list file for panel data.
string
Semicolon-separated list of Isofox functions to run
string
TRANSCRIPT_COUNTS;ALT_SPLICE_JUNCTIONS;FUSIONS;RETAINED_INTRONS
Reference data files and options required for the workflow.
Name of genome reference.
string
Version of reference genome.
string
Type of reference genome.
string
Do not load the iGenomes reference config.
boolean
true
Do not load igenomes.config
when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in igenomes.config
.
The base path to the igenomes reference files
string
s3://ngi-igenomes/igenomes/
The base path to the HMF genome reference files
string
https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes
Path to HMF data.
string
Path to panel data.
string
Path to VIRUSBreakend database (directory or tarball).
string
Path to reference genome FASTA.
string
Path to reference genome FAI.
string
Path to reference genome dict.
string
Path to reference genome bwa-mem2 index.
string
Path to reference genome GRIDSS index.
string
Path to reference genome STAR index.
string
Path to reference genome GTF.
string
Path to HLA slice BED file.
string
^\S+\.bed$
Parameters used to describe centralised config profiles. These should not be edited.
Git commit id for Institutional configs.
string
master
Base directory for Institutional configs.
string
https://raw.githubusercontent.com/nf-core/configs/master
If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.
Institutional config name.
string
Institutional config description.
string
Institutional config contact information.
string
Institutional config URL link.
string
Less common options for the pipeline, typically set in a config file.
Display version and exit.
boolean
Method used to save pipeline results to output directory.
string
The Nextflow publishDir
option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See Nextflow docs for details.
Email address for completion summary, only when pipeline fails.
string
^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$
An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.
Send plain-text email instead of HTML.
boolean
Do not use coloured log outputs.
boolean
Incoming hook URL for messaging service
string
Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.
Boolean whether to validate parameters against the schema at runtime
boolean
true
Base URL or local path to location of pipeline test dataset files
string
https://raw.githubusercontent.com/nf-core/test-datasets/oncoanalyser